TruQuick™ HIV p24
TruQuick HIV P24 is a rapid immunoassay for the qualitative detection of p24 antigen to HIV 1 in whole blood, serum or plasma.
The HIV p24 antigen is a small piece of protein that is found on the capsule of the HIV virus. When a person is infected with HIV, these bits of protein can be found floating in the blood. The HIV p24 antigen rapid test is the test that detects these bits of protein. This test was first developed as a HIV screening test but rapidly ran out of favor due to the development of more advanced NAAT tests.1 The window period for p24 testing is also very small. This test alone is only accurate for between three and six weeks post exposure.2 So it is a test with very limited applications unless combined with HIV p24 antigen test. The presence of p24 antigen in the blood indicated a recent HIV infection.3 TruQuick HIV P24 is a rapid test to qualitatively detect the presence of p24 antigen to HIV 1 in whole blood, serum or plasma specimen. The test utilizes latex conjugate HIV p24 antibody to selectively detect p24 antigen to the HIV type 1 in whole blood, serum or plasma.
- Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it as soon as possible.
- Place the cassette on a clean and level surface.
- For Serum or Plasma specimen: Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 µL) to the specimen area, then add 1 drop of Buffer (approximately 40 µL) and start the timer. See illustration below.
- For Venipuncture Whole Blood specimen: Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50 µL) to the specimen area, then add 2 drops of Buffer (approximately 80 µL) and start the timer. See illustration below.
- For Fingerstick Whole Blood specimen:
- To use a capillary tube: Fill the capillary tube and transfer approximately 50 µL of fingerstick whole blood specimen to the specimen area of Test Cassette, then add 2 drops of Buffer (approximately 80 µL) and start the timer. See illustration below.
- To use hanging drops: Allow 2 hanging drops of fingerstick whole blood specimen (approximately 50 µL) to fall into the specimen area of Test Cassette, then add 2 drops of Buffer (approximately 80 µL) and start the timer. See illustration below.
- Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes
- Blacklist of English teachers suspected of having AIDS pursued. This image of Randall L Tobias is used in a Korean news article suggesting that foreign English teachers residing in Korea are at risk for AIDS. Accessed 16 Feb 2010.
- Keeping blood transfusions safe: FDA’s multi-layered protections for donated blood. US FDA Retrieved 12 Oct 2013.
- FDA approves first nucleic acid test (NAT) systems to screen plasma for human immunodeficiency virus (HIV) and hepatitis C virus (HCV).