S100B Human ELISA
S-100B protein is a member of highly homologous acidic calcium and zinc binding proteins family (S100 family) that possess two EF-hand motifs. S100B is a bb homodimer with the molecular weight 21 kDa. S100B is abundant in the nervous system where it is predominantly expressed in astrocytes, oligodendrocytes and Schwann cells. S100B is released from brain tissue into cerebrospinal fluid (CSF) and blood following brain damage of different origin, i.e., brain infarction, intracerebral or subarachnoidal hemorrhage, neurotrauma and large intracerebral tumors. It is documented a relationship between degree of cell damage and S100B levels in CSF. Also patients with progressive melanoma disease show elevated serum concentrations of S100B.
The RD192090100R Human S100B ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human S100B in serum, heparin plasma and cerebrospinal fluid.
In the BioVendor Human S100B ELISA, standards, quality controls and samples are incubated in microplate wells pre-coated with polyclonal anti-cow S100B antibody. After 120 minutes incubation and washing, biotin labelled monoclonal anti-human S100B antibody is added to the wells and incubated for 60 minutes with captured S100B. After another washing, Streptavidin- HRP Conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the Substrate Solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of S100B. A standard curve is constructed by plotting absorbance values against concentrations of Standards, and concentrations of unknown samples are determined using this standard curve.
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Clinical Application
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Summary of protocol
– Bayram H, Hidiroglu M, Cetin L, Kucuker A, Iriz E, Uguz E, Saglam F, Sener E. Comparing S-100 beta protein levels and neurocognitive functions between patients undergoing on-pump and off-pump coronary artery bypass grafting. J Surg Res. 2013 Jun 15;182 (2):198-202
– Brouns R, De Vil B, Cras P, De Surgeloose D, Marien P, De Deyn PP. Neurobiochemical Markers of Brain Damage in Cerebrospinal Fluid of Acute Ischemic Stroke Patients. Clin Chem. 2009 Dec 3;
– Büyükaslan H, Kandemir SB, Asoğlu M, Kaya H, Gökdemir MT, Karababa İF, Güngörmez F, Kılıçaslan F, Şavik E. Evaluation of oxidant, antioxidant, and S100B levels in patients with conversion disorder. Neuropsychiatric Disease and T. July 2016;
– Cai XY, Lu L, Wang YN, Jin C, Zhang RY, Zhang Q, Chen QJ, Shen WF. Association of increased S100B, S100A6 and S100P in serum levels with acute coronary syndrome and also with the severity of myocardial infarction in cardiac tissue of rat models with ischemia-reperfusion injury. Atherosclerosis. 2011 Aug;217 (2):536-42
– Chen X, Chen Y, Wei Q, Ou R, Cao B, Zhao B, Shang HF. Assessment of a multiple biomarker panel for diagnosis of amyotrophic lateral sclerosis. BMC Neurol. 2016;16:173
– Cicek IE, Cicek E, Kayhan F, Uguz F, Erayman I, Kurban S, Yerlikaya FH, Kaya N. The roles of BDNF, S100B, and oxidative stress in interferon-induced depression and the effect of antidepressant treatment in patients with chronic viral hepatitis: a prospective study. J Psychosom Res. 2014 Mar;76 (3):227-32
– Dilek A, Alacam H, Ulger F, Bedir A, Ulus A, Murat N, Okuyucu A, Polat F. Comparison of predictive powers of S100B and cell-free plasma DNA values in intensive care unit patients with intracranial hemorrhage. J Crit Care. 2013 Oct;28 (5):883.e1-7
– Esquivel AO, Sherman SS, Bir CA, Lemos SE. The Interaction of Intramuscular Ketorolac (Toradol) and Concussion in a Rat Model. Ann Biomed Eng. 2017 Jun;45(6):1581-1588. doi: 10.1007/s10439-017-1809-5
– Friel LA, Romero R, Edwin S, Nien JK, Gomez R, Chaiworapongsa T, Kusanovic JP, Tolosa JE, Hassan SS, Espinoza J. The calcium binding protein, S100B, is increased in the amniotic fluid of women with intra-amniotic infection/inflammation and preterm labor with intact or ruptured membranes. J Perinat Med. 2007;35 (5):385-93
– Hosseinzadeh S, Zahmatkesh M, Zarrindast MR, Hassanzadeh GR, Karimian M, Sarrafnejad A. Elevated CSF and plasma microparticles in a rat model of streptozotocin-induced cognitive impairment. Behav Brain Res. 2013 Sep 12;256C:503-511
– Hsu AA, Fenton K, Weinstein S, Carpenter J, Dalton H, Bell MJ. Neurological injury markers in children with septic shock. Pediatr Crit Care Med. 2008 May;9 (3):245-51
– Hu S, Dong HL, Li YZ, Luo ZJ, Sun L, Yang QZ, Yang LF, Xiong L. Effects of remote ischemic preconditioning on biochemical markers and neurologic outcomes in patients undergoing elective cervical decompression surgery: a prospective randomized controlled trial. J Neurosurg Anesthesiol. 2010 Jan;22 (1):46-52
– Ito M, Bolati K, Kinjo T, Ichimura K, Furuta A, McLoughlin DM, Suzuki T, Arai H. Electroconvulsive stimulation transiently enhances the permeability of the rat blood-brain barrier and induces astrocytic changes. Brain Res Bull. 2017 Jan;128:92-97
– Jesse S, Steinacker P, Cepek L, von Arnim CA, Tumani H, Lehnert S, Kretzschmar HA, Baier M, Otto M. Glial fibrillary acidic protein and protein S-100B: different concentration pattern of glial proteins in cerebrospinal fluid of patients with Alzheimer’s disease and Creutzfeldt-Jakob disease. J Alzheimers Dis. 2009 Jul;17 (3):541-51
– Kim JK, Kim SG, Kim HJ, Song YR. Serum S100B protein is associated with depressive symptoms in patients with end-stage renal disease. Clin Biochem. 2012 Dec;45 (18):1573-7
– Kwon BK, Stammers AM, Belanger LM, Bernardo A, Chan D, Bishop CM, Slobogean GP, Zhang H, Umedaly H, Giffin M, Street J, Boyd MC, Paquette SJ, Fisher CG, Dvorak MF. Cerebrospinal Fluid Inflammatory Cytokines and Biomarkers of Injury Severity in Acute Human Spinal Cord Injury. J Neurotrauma. 2009 Dec 28;
– Kwon BK, Streijger F, Fallah N, Noonan V, Belanger LM, Ritchie L, Paquette SJ, Ailon T, Boyd MC, Street J, Fisher CG, Dvorak MF. Cerebrospinal Fluid Biomarkers to Stratify Injury Severity and Predict Outcome in Human Traumatic Spinal Cord Injury. J Neurotrauma. 2016 Jun 27;
– Lasn H. The principal inferior Olivary Nucleus in Aging and Alzheimer´s disease. 2006;
– Misu T, Takano R, Fujihara K, Takahashi T, Sato S, Itoyama Y. Marked increase in cerebrospinal fluid glial fibrillar acidic protein in neuromyelitis optica: an astrocytic damage marker. J Neurol Neurosurg Psychiatry. 2009 May;80 (5):575-7
– Miyazaki I, Asanuma M, Murakami S, Takeshima M, Torigoe N, Kitamura Y, Miyoshi K. Targeting 5-HT1A receptors in astrocytes to protect dopaminergic neurons in parkinsonian models. Neurobiol Dis. 2013 Nov;59:244-56
– Morera-Fumero AL, Abreu-Gonzalez P, Henry-Benitez M, Yelmo-Cruz S, Diaz-Mesa E. Summer/winter changes in serum S100B protein concentration as a source of research variance. J Psychiatr Res. 2013 Jun;47 (6):791-5
– Morera-Fumero AL, Díaz-Mesa E, Abreu-Gonzalez P, Fernandez-Lopez L, Cejas-Mendez MD. Day/night changes in serum S100B protein concentrations in acute paranoid schizophrenia. Prog Neuropsychopharmacol Biol Psychiatry. 2017 Apr 3;75:207-212. doi: 10.1016/j.pnpbp.2017.02.007
– Ozaki S, Kurata M, Kumon Y, Matsumoto S, Tagawa M, Watanabe H, Ohue S, Higaki J, Ohnishi T. Plasma thrombin-cleaved osteopontin as a potential biomarker of acute atherothrombotic ischemic stroke. Hypertens Res. 2016 Aug 25;
– Pawluski JL, Galea LA, Brain U, Papsdorf M, Oberlander TF. Neonatal S100B protein levels after prenatal exposure to selective serotonin reuptake inhibitors. Pediatrics. 2009 Oct;124 (4):e662-70
– Pranzatelli MR, Tate ED, McGee NR, Verhulst SJ. CSF neurofilament light chain is elevated in OMS (decreasing with immunotherapy) and other pediatric neuroinflammatory disorders. J Neuroimmunol. 2014 Jan 15;266 (1-2):75-81
– Rogers LK, Graf AE, Bhatia A, Leonhart KL, Oza-Frank R. Associations between Maternal and Infant Morbidities and sRAGE within the First Week of Life in Extremely Preterm Infants. PLoS One. 2013;8 (12):e82537
– Serarslan Y, Bal R, Altug ME, Kontas T, Melek IM. Caffeic acid phenethyl ester decreases the level of S-100B protein after middle cerebral artery [correction for after] occlusion in rabbits. Pak J Pharm Sci. 2009 Jul;22 (3):313-6
– Stammet P, Wagner DR, Gilson G, Devaux Y. Modeling serum level of s100beta and bispectral index to predict outcome after cardiac arrest. J Am Coll Cardiol. 2013 Aug 27;62 (9):851-8
– Steiner J, Bielau H, Bernstein HG, Bogerts B, Wunderlich MT. Increased cerebrospinal fluid and serum levels of S100B in first-onset schizophrenia are not related to a degenerative release of glial fibrillar acidic protein, myelin basic protein and neurone-specific enolase from glia or neurones. J Neurol Neurosurg Psychiatry. 2006 Nov;77 (11):1284-7
– Uzbay T, Goktalay G, Kayir H, Eker SS, Sarandol A, Oral S, Buyukuysal L, Ulusoy G, Kirli S. Increased plasma agmatine levels in patients with schizophrenia. J Psychiatr Res. 2013 Aug;47 (8):1054-60
– Xiao L, Sun W, Lan W, Xiong Y, Duan Z, Zhang Z, Fan W, Xu L, Xie X, Ma N, Ye R, Xu G, Liu X, Zhu W. Correlation between cerebral microbleeds and S100B/RAGE in acute lacunar stroke patients. J Neurol Sci. 2014 Mar 11;