Copper Assay Kit (Cu)
Product Features
The assay kit is for determination of Copper (Cu). As one of the most important trace elements, Copper(Cu) is a part of many metal enzymes and participate in synthesizing melanin, collagen. The decrease of copper may cause hypogenesis, anaemia of sex of cellule low pigment. The acute toxicity of copper can cause acute renal failure and gastro-enteritis.
Assay Principle
Acidic conditions
CER-Cu2+ → CER + Cu2+
Acidic conditions
Albumin-Cu2+ → Albumin + Cu2+
Cu2++VC(reduced) → Cu+ + VC(oxidized)
Cu++3,5-DiBr-PAESA → Complex(blue)
Calculate the copper concectration by the adsorbance at 600 nm.
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References
- Abc,S.Yamashita, A. Noma, Sensitive, direct colorimetric assay for copper in serum, Clin Chem, 35,552-554(1989)
- Katarzyna Zawistowska. Copper chelate with 2pyridylazo ligands as test probes for characterization of micellar effects. COLLOIDS AND SURFACES, 2008, 315: 259-267
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