Borrelia IgG VIsE ELISA
Enzyme immunoassay for measurement of IgG antibodies against Borrelia burgdorferi in human serum, plasma and CSF. Infections with all three B. burgdorferi subspecies (garinii, afzelii and sensu strictu) are detected.
Test Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgG. After the substrate reaction the intensity of the developed color is proportional to the amount of detected IgG-specific antibodies. Results of samples can be determined directly using the standard curve or calibrator standard.
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References
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