West Nile Virus Antibodies
Enzyme ImmunoAssay (ELISA) for the qualitative determination of antibodies to West Nile Virus in human plasma and sera. The product is intended mostly for the follow-up of West Nile Virus infection.
West Nile Virus is an arbovirus and is transmitted to people through complex life cycles involving birds and mosquitoes. Each infection is most often not trasmitted from person to person.
Most peolple who are infected with west Nile Virus will not have any symptoms. Clinician estimate that 20% of the people who become infected will develop West Nile fever: mild symptoms, fever, headache, and body aches, occasionally with a skin rash on the trunk of the body and swollen lymph glands. 1 in 150 west Nile Virus infections(< 1%) result in meningitis or encephalitis. The case fatality rates for WNV are similar to St.Louis encephalitis virus and western equine encephalitis virus: 5-15%.
Microplates are coated with an highly purified immunodominant West Nile virus antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti WNV antibodies are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti WNV are detected by the addition of a mix of both anti hIgG antibody, labeled with peroxidase (HRP) and anti hIgM antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of WNV antibodies present in the sample.
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