TruQuick™ Zika
TruQuick Zika is a rapid chromatographic immunoassay for the qualitative detection of NS1 antigen of Zika virus in whole blood, serum, or plasma as an aid in the diagnosis of Zika infections.
Zika virus (ZIKV) is a member of the virus family Flaviviridae.1 It is spread by daytime-active Aedes mosquitoes, such as A. aegypti and A. albopictus.1 Its name comes from the Zika Forest of Uganda, where the virus was first isolated in 1947.2 Zika virus is related to the dengue, yellow fever, Japanese encephalitis, and West Nile viruses.4 Since the 1950s, it has been known to occur within a narrow equatorial belt from Africa to Asia. From 2007 to 2016, the virus spread eastward, across the Pacific Ocean to the Americas, leading to the 2015–16 Zika virus epidemic.
The infection, known as Zika fever or Zika virus disease, often causes no or only mild symptoms, similar to a very mild form of dengue fever.1 While there is no specific treatment, paracetamol (acetaminophen) and rest may help with the symptoms.3 As of 2016, the illness cannot be prevented by medications or vaccines.3 Zika can also spread from a pregnant woman to her fetus. This can result in microcephaly, severe brain malformations, and other birth defects.4, 5 Zika infections in adults may result rarely in Guillain–Barré syndrome.6
TruQuick Zika is a rapid test that utilizes Zika NS1 antibody-coated colored particles for the detection of Zika NS1 antigen in whole blood, serum, or plasma.
- Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it within 1 hour.
- Place the cassette on a clean and level surface.
- For Serum or Plasma specimen:
- Hold the dropper vertically and transfer 1 drop of serum or plasma (approximately 25 μL) to the specimen area, then add 1 drop (approx. 40 μL) of Buffer and start the timer. See illustration below. For Venipuncture
- Whole Blood specimen:
- Hold the dropper vertically and transfer 2 drops of whole blood (approximately 50 μL) to the specimen area, then add 1 drop of Buffer (approximately 40 μL), and start the timer. See illustration below.
- For Fingerstick Whole Blood specimen:
- To use a capillary tube: Fill the capillary tube and transfer approximately 50 μL of fingerstick whole blood specimen to the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- To use hanging drops: Allow 2 hanging drops of fingerstick whole blood specimen (approximately 50 μL) to fall into the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- For Serum or Plasma specimen:
- Wait for the colored line(s) to appear. Read results at 15 minutes. Do not interpret the result after 20 minutes.
- Malone RW, Homan J, Callahan MV, et al. Zika Virus: Medical countermeasure development challenges. PLoS Negl Trop Dis. 2016;10(3):e0004530.doi:10.1371/journal.pntd.0004530..
- Sikka V, Chattu VK, Popli RK, et al. The emergence of zika virus as a global health security threat: A review and a consensus statement of the INDUSEM Joint Working Group (JWG). J Global Infect Dis. 8(1):3–15. 11 February 2016.
- Symptoms, diagnosis, and treatment. Zika virus. Atlanta: Centers for Disease Control and Prevention. 3 March 2016.
- Rasmussen SA, Jamieson DJ, Honein MA, Petersen LR. Zika virus and birth defects — reviewing the evidence for causality. N Engl J Med. 374:1981–1987. 13 April 2016.
- CDC concludes Zika causes microcephaly and other birth defects. CDC. 13 April 2016. 6. Zika virus microcephaly and Guillain–Barré syndrome situation report. WHO. 7 April 2016.