TruQuick™ RSV

TruQuick RSV is a qualitative, lateral flow chromatographic immunoassay for the detection of Respiratory Syncytial Virus antigen in nasopharyngeal swab or nasal aspirate specimens.


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Product Catalog No: TQ5320 Pack Size: 20 Tests

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Summary

Respiratory Syncytial Virus (RSV), which causes infection of the lungs and breathing passages, is a major cause of respiratory illness in young children. In adults, it may only produce symptoms of a common cold, such as a stuffy or runny nose, sore throat, mild headache, cough, fever, and a general feeling of being ill. But in premature babies and kids with diseases that affect the lungs, heart, or immune system, RSV infections can lead to other more serious illnesses.1 RSV is highly contagious and can be spread through droplets containing the virus when someone coughs or sneezes. It also can live on surfaces (such as countertops or doorknobs) and on hands and clothing, so it can be easily spread when a person touches something contaminated. RSV can spread rapidly through schools and childcare centers. Babies often get it when older kids carry the virus home from school and pass it to them. Almost all kids are infected with RSV at least once by the time they’re 2-3 years old.2 RSV infections often occur in epidemics that last from late fall through early spring. Respiratory illness caused by RSV — such as bronchiolitis or pneumonia — usually lasts about a week, but some cases may last several weeks.

Test Procedure
  1. Remove the Test Cassette from the sealed foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed immediately after opening the foil pouch.
  2. Place the Extraction Tube in the workstation. Hold the Extraction Reagent bottle upside down vertically. Squeeze the bottle and let the solution drop into the Extraction Tube freely without touching the edge of the tube. Add 10 drops of solution (approx. 400 μL) to the Extraction Tube.
  3. Place the swab specimen in the Extraction Tube. Rotate the swab for approximately 10 seconds while pressing the head against the inside of the tube to release the antigen in the swab.
  4. Remove the swab while squeezing the swab head against the inside of the Extraction Tube as you remove it to expel as much liquid as possible from the swab. Discard the swab in accordance with your biohazard waste disposal protocol.
  5. Fit the dropper tip on top of the Extraction Tube. Place the Test Cassette on a clean and level surface.
  6. Add 3 drops of the solution (approx.120 μL) to the sample well and then start the timer. Read the result at 15 minutes. Do not interpret the result after 20 minutes.
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    References
    1. Glezen WP, Taber LH, Frank AL, Kasel JA. Risk of primary infection and reinfection with respiratory syncytial virus. American journal of diseases of children. 1986;(1960)140(6): 543–6.
    2. Hall CB, Weinberg GA, Iwane MK, Blumkin AK, Edwards KM, Staat MA, et al. The burden of respiratory syncytial virus infection in young children. New Engl J of Med. 2009;360(6):588–98.
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