TruQuick™ Hb+Hb/Hp Combo

The TruQuick Hb+Hb/Hp Combo is a sandwich immunoassay. It is a combo test consisting of 2 individual tests, a hemoglobin (Hb) test and a hemoglobin-haptoglobin (Hb/Hp) complex test to selectively identify human Hb and/or Hb/Hp complexes in test samples. Each test contains a nitrocellulose membrane strip with an immobilized mouse anti- Hb -antibody on the test zone (T). The Hb test has a pad containing poly anti-Hb colloidal gold and mouse IgE colloidal gold. The Hb/Hp complex test has a pad containing poly anti-Hp colloidal gold and mouse IgE colloidal gold. A goat-anti-mouse antibody is immobilized in the control line zone (C) on the nitrocellulose membrane. 90 to 120μl specimen (buffer mixed) specimen (containing the respective analytes, Hb and Hb/Hp complexes) is added onto the respective specimen pad. The analytes (i.e. Hb and/or Hb/Hp complex) in the collected specimen couple with the mouse anti-Hb-antibody or anti-Hp-antibody of the gold conjugate pad thus forming antibody – antigen – colloidal gold complexes. While the liquid is moving along the membrane it transports these complexes by capillary action. When the antibody – antigen – colloidal gold complexes are transported across the membrane and reach the respective immobilized mouse anti- Hb-antibody on the membrane, they are trapped and will form a sandwich complex consisting of: immobilized antibody – antigen – antibody – colloidal gold for Hb and/ or Hb/Hp complex respectively. Only when the applied specimen sample contains a certain concentration of Hb or Hb/Hp complex, the formation of this sandwich complex will result in a visible magenta color band in the respective test zones (T) of the membrane. The colored C-line will always appear if the test has been performed correctly. The test sensitivity for Hb is 50ng/ml and Hb/Hp is 50ng/ml.