TruQuick™ H.pylori Ab
TruQuick H. pylori Ab is a rapid immunoassay for the qualitative detection of antibodies to H. pylori in whole blood, serum, or plasma to aid in the diagnosis of H. pylori infection.
H. pylori is a small, spiral-shaped bacterium that lives in the surface of the stomach and duodenum. It is implicated in the etiology of a variety of gastrointestinal diseases, including duodenal and gastric ulcer, non-ulcer dyspepsia and active and chronic gastritis.1, 2 Both invasive and non-invasive methods are used to diagnose H. pylori infection in patients with symptoms of gastrointestinal disease. Specimen dependent and costly invasive diagnostic methods include gastric or duodenal biopsy followed by urease testing (presumptive), culture, and/or histologic staining.3 Non-invasive techniques include the urea breath test, which requires expensive laboratory equipment and moderate radiation exposure, and serological methods.4, 5 Individuals infected with H. pylori develop antibodies which correlate strongly with histologically confirmed H. pylori infection.6- 8 TruQuick H. pylori Ab is a simple test that utilizes a combination of H. pylori antigen-coated particles and antihuman IgG to qualitatively and selectively detect H. pylori antibodies in whole blood, serum, or plasma.
- Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it as soon as possible.
- Place the cassette on a clean and level surface.
- For Serum or Plasma specimen:
- Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 75 μL) to the specimen well of Test Cassette and start the timer. See illustration below.
- For Venipuncture Whole Blood specimen:
- Hold the dropper vertically and transfer 3 drops of whole blood (approximately 75 μL) to the specimen well, then add 1 drop of Buffer (approximately 40 μL), and start the timer. See illustration below.
- For Fingerstick Whole Blood specimen:
- To use a capillary tube: Fill the capillary tube and transfer approximately 75 μL of fingerstick whole blood specimen to the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- To use hanging drops: Allow 3 hanging drops of fingerstick whole blood specimen (approximately 75 μL) to fall into the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- For Serum or Plasma specimen:
- Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes.
- Marshall BJ, McGechie DB, Rogers PAR, Glancy, RG. Pyloric Campylobacter infection and gastroduodenal disease. Med J Australia 1985;149:439-44.
- Soll AH. Pathogenesis of peptic ulcer and implications for therapy. N Engl J Med 1990;322:909-16.
- Hazell SL, et al. Campylobacter pyloridis and gastritis I: Detection of urease as a marker of bacterial colonization and gastritis. Am J Gastroenterol 1987;82(4):292-96.
- Loffeld RJLF, et al. Usefulness of several commercial enzyme-linked immunoassays for detection of Helicobacter pylori infection in clinical medicine. Euro J Gastroen Hepa. 1993;333-37.
- Cutler AF, et al. Accuracy of invasive and non-invasive tests to diagnose Helicobacter pylori infection. Gastroenterology. 1995;109:136-141.
- Ansorg R, Von Recklinghausen G, Pomarius R, Schmid EN. Evaluation of techniques for isolation, subcultivation and preservation of Helicobacter pylori. J Clin Micro 1991;29:51 53.
- Pronovost AP, Rose SL, Pawlak J, Robin H, Schneider R. Evaluation of a new immunodiagnostic assay for Helicobacter pylori antibody detection: Correlation with histopathological and microbiological results. J Clin Micro 1994;32:46-50.
- Megraud F, Bassens-Rabbe MP, Denis F, Belbouri A, Hoa DQ. Seroepidemiology of Campylobacter pylori infection in various populations. J Clin Micro 1989;27:1870-3.