TruQuick™ Dengue NS1
TruQuick Dengue NS1 is a rapid immunoassay for the qualitative detection of NS1 antigen of Dengue virus in whole blood, serum, or plasma as an aid in the diagnosis of Dengue infections. A positive test should be confirmed with alternative testing and clinical findings.
Dengue is a flavivirus, transmitted by Aedes aegypti and Aedes albopictus mosquitoes. It is widely distributed throughout the tropical and subtropical areas of the world,1 and causes up to 100 million infections annually.2 Classic Dengue infection is characterized by a sudden onset of fever, intense headache, myalgia, arthralgia and rash. NS1 is one of seven Dengue Virus nonstructural proteins which are thought to be involved in viral replication. NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown. Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.
TruQuick Dengue NS1 is a rapid test that utilizes a combination of Dengue antibody-coated colored particles for the detection of Dengue NS1 antigen in whole blood, serum, or plasma.
- Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it within 1 hour.
- Place the cassette on a clean and level surface.
- For Serum or Plasma specimen:
- Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 75 μL) to the specimen area, and start the timer. See illustration below.
- For Venipuncture Whole Blood specimen:
- Hold the dropper vertically and transfer 3 drops of whole blood (approximately 75 μL) to the specimen area, then add 1 drops of Buffer (approximately 40 μL) and start the timer. See illustration below.
- For Fingerstick Whole Blood specimen:
- To use a capillary tube: Fill the capillary tube and transfer approximately 75 μL of fingerstick whole blood specimen to the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- To use hanging drops: Allow 3 hanging drops of fingerstick whole blood specimen (approximately 75 μL) to fall into the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- For Serum or Plasma specimen:
- Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes.
- Halstead SB. Selective primary health care: strategies for control of disease in the developing world: XI, Dengue. Rev Infect Dis. 1984;6:251-264.
- Halstead SB. Pathogenesis of dengue: challenges to molecular biology. Science 1988;239:476-481.