TruQuick™ Dengue IgG/IgM
TruQuick Dengue IgG/IgM is a rapid immunoassay for the qualitative detection of IgG and IgM antibodies to Dengue virus in whole blood, serum, or plasma as an aid in the diagnosis of primary and secondary Dengue infections.
SUMMARY AND EXPLANATION OF THE TEST Dengue is a flavivirus, transmitted by Aedes aegypti and Aedes albopictus mosquitoes. It is widely distributed throughout the tropical and subtropical areas of the world,1 and causes up to 100 million infections annually.2 Classic Dengue infection is characterized by a sudden onset of fever, intense headache, myalgia, arthralgia and rash. Primary Dengue infection causes IgM antibodies to increase to a detectable level in 3 to 5 days after the onset of fever. IgM antibodies generally persist for 30 to 90 days.3 Most Dengue patients in endemic regions have secondary infections,4 resulting in high levels of specific IgG antibodies prior to or simultaneous with IgM response.5 Therefore, the detection of specific anti-Dengue IgM and IgG antibodies can also help to distinguish between primary and secondary infections.
The TruQuick Dengue IgG/IgM is a rapid test that utilizes a combination of Dengue antigen-coated colored particles for the detection of IgG and IgM Dengue antibodies in whole blood, serum, or plasma.
- Bring the pouch to room temperature before opening. Remove the Test Cassette from the sealed pouch and use it within one hour.
- Place the Test Cassette on a clean and level surface.
- For Serum or Plasma Specimens:
- To use a dropper: Hold the dropper vertically, draw the specimen up to the Fill Line (approximately 5 μL), and transfer the specimen to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. Avoid trapping air bubbles in the specimen well.
- To use a micropipette: Pipette and dispense 5 μL of specimen to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer.
- For Whole Blood (Venipuncture/Fingerstick) Specimens:
- To use a dropper: Hold the dropper vertically, draw the specimen about 1 cm above the Fill Line, and transfer 1 drop of whole blood (approximately 10 μL) to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 uL) and start the timer.
- To use a micropipette: Pipette and dispense 10 μL of whole blood to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer.
- For Serum or Plasma Specimens:
- Wait for the colored line(s) to appear. The test result should be read at 10 minutes. Do not interpret the result after 20 minutes.
- Halstead SB. Selective primary health care: strategies for control of disease in the developing world: XI, Dengue. Rev Infect Dis. 1984;6:251-264.
- Halstead SB. Pathogenesis of dengue: challenges to molecular biology. Science. 1988;239:476-481.
- Ruechusatsawat K. et al. Daily observation of antibody levels among dengue patients detected by enzyme-linked immunosorbent assay (ELISA). Japanese J Trop Med Hygiene. 1994;22:9-12.
- Lam SK. Dengue haemorrhagic fever. Rev Med Micro. 1995;6:39-48.
- Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. 2nd edition. Geneva: WHO.
- Yamada K, et al. Antibody responses determined for Japanese dengue fever patients by neutralization and hemagglutination inhibition assays demonstrate cross-reactivity between dengue and Japanese encephalitis viruses. Clin Diagn Lab Immunol. 2003 Jul; 10(4):725-8.
- Dobler G, et al. Cross reactions of patients with acute dengue fever to tick-borne encephalitis. Wien Med Wochenschr (in German). 1997;147(19-20):463-4.
- Makino Y, et al. Studies on serological cross-reaction in sequential flavivirus infections. Microbiol Immunol. 1994;38(12):951-5.