TruQuick™ DENG IgG/IgM/NS1
TruQuick DENG IgG/IgM/NS1 is a rapid chromatographic immunoassay for the qualitative detection of NS1 antigen and IgG and IgM antibodies of Dengue virus in whole blood, serum, or plasma as an aid in the diagnosis of Dengue infections.
Dengue is a flavivirus, transmitted by Aedes aegypti and Aedes albopictus mosquitoes. It is widely distributed throughout the tropical and subtropical areas of the world,1 and causes up to 100 million infections annually.2 Classic Dengue infection is characterized by a sudden onset of fever, intense headache, myalgia, arthralgia and rash.
Primary Dengue infection causes IgM antibodies to increase to a detectable level in three to five days after the onset of fever. IgM antibodies generally persist for 30 to 90 days.3 Most Dengue patients in endemic regions have secondary infections,4 resulting in high levels of specific IgG antibodies prior to or simultaneous with IgM response.5 Therefore, the detection of specific anti- Dengue IgM and IgG antibodies can also help to distinguish between primary and secondary infections.
- Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it within 1 hour.
- Place the cassette on a clean and level surface.
- a. For Serum or Plasma specimen: For NS1:
- Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 75 μL) to the specimen area, and start the timer. See illustration below. For IgG/IgM:
- To use a dropper: Hold the dropper vertically, draw the specimen up to the Fill Line (approximately 5 μL) and transfer the specimen to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. Avoid trapping air bubbles in the specimen well.
- To use a micropipette: Pipette and dispense 5 μL of serum or plasma to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer.
- b. For Whole Blood (Venipuncture/Fingerstick) specimen: For NS1:
- To use a dropper: Hold the dropper vertically and transfer 3 drops of whole blood (approximately 75 μL) to the specimen area, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- To use a capillary tube: Fill the capillary tube and transfer approximately 75 μL of fingerstick whole blood specimen to the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- To use hanging drops: Allow 3 hanging drops of fingerstick whole blood specimen (approximately 75 μL) to fall into the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- For IgG/IgM: To use a dropper: Hold the dropper vertically, draw the specimen above 1 cm above the Fill Line, and transfer 1 drop of whole blood (approximately 10 μL) to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- To use a micropipette: Pipette and dispense 10 μL of whole blood to the specimen well of the Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- Read the results at 10 minutes, do not interpret the results after 20 minutes.
- Halstead SB. Selective primary health care: strategies for control of disease in the developing world: XI, Dengue. Rev Infect Dis. 1984;6:251-264.
- Halstead SB. Pathogenesis of dengue: challenges to molecular biology. Science 1988;239:476-481.
- Ruechusatsawat K, et al. Daily observation of antibody levels among dengue patients detected by enzyme-linked immunosorbent assay (ELISA). Japanese, J Trop Med Hygiene. 1994;22:9-12.
- Lam SK. Dengue haemorrhagic fever. Rev Med Micro. 1995;6:39-48.
- Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. 2nd ed. Geneva: WHO.
- Yamada K, et al. Antibody responses determined for Japanese dengue fever patients by neutralization and hemagglutination inhibition assays demonstrate crossreactivity between dengue and Japanese encephalitis viruses. Clin Diagn Lab Immunol. 2003 Jul;10(4):725-8.
- Dobler G, et al. Cross reactions of patients with acute dengue fever to tick-borne encephalitis. Wien Med Wochenschr (in German). 1997;147(19-20):463-4.
- Makino Y, et al. Studies on serological cross-reaction in sequential flavivirus Microbiol Immunol. 1994;38(12):951-5.