TruQuick™ CK-MB
Summary
TruQuick CK-MB is a rapid chromatographic immunoassay for the qualitative detection of human CK-MB in whole blood, serum or plasma as an aid in the diagnosis of myocardial infarction (MI).
Test Procedure
- Bring the pouch to room temperature before opening it. Remove the Test Cassette from the sealed pouch and use it within one hour.
- Place the cassette on a clean and level surface.
- For Serum or Plasma specimen:
- Hold the dropper vertically and transfer 2 drops of serum or plasma (approximately 50 μL) to the specimen area, then add 1 drops of Buffer (approximately 40 μL), and start the timer. See illustration below.
- For Venipuncture Whole Blood specimen:
- Hold the dropper vertically and transfer 3 drops of whole blood (approximately 75 μL) to the specimen area, then add 1 drops of Buffer (approximately 40 μL), and start the timer. See illustration below.
- For Fingerstick Whole Blood specimen:
- To use a capillary tube: Fill the capillary tube and transfer approximately 75 μL of fingerstick whole blood specimen to the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- To use hanging drops: Allow 3 hanging drops of fingerstick whole blood specimen (approximately 75 μL) to fall into the specimen area of Test Cassette, then add 1 drop of Buffer (approximately 40 μL) and start the timer. See illustration below.
- For Serum or Plasma specimen:
- Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes
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References
- Apple FS, Preese LM. Creatine kinase-MB: detection of myocardial infarction and monitoring reperfusion. J Clin Immunoassay. 1994;17:24-9.
- Lee TH, Goldman L. Serum enzyme assays in the diagnosis of acute myocardial infarction. Ann Intern Med. 1986;105:221-233.
- Kallner A, Sylven C, Brodin U, et al. Early diagnosis of acute myocardial infarction; a comparison between chemical predictors. Scand J Clin Lab Invest. 1989;49:633-9.