Trefoil Factor 2 Human ELISA
Trefoil factor 2 (TFF2) (PSP – pancreatic spasmolytic polypeptide) is a small secreted protein with a molecular weight of 12 kDa. It belongs to the TFF protein family that is characterized by a clover leaf–like disulphide structure named the TFF domain, which is created by 6 cysteines forming three intramolecular bonds. TFF2 contains two trefoil domains and has a very compact structure which may account for its extremely high resistance against the harsh environment in the gastrointestinal tract. The most abundant expression of TFFs is found in GI tract (especially in stomach, colon and pancreas), where they are co-localised with mucins. TFF2 is usually co-localised with MUC6 and probably mediates mucin cross-linking and stabilization of the mucin layer as do the other members of the TFF family. A study examining people with Crohn´s disease and inflammatory bowel disease showed that TFF2 level in serum is increased during the inflammatory state. Another study found that TFF2 levels are high in septic patients and that the level correlates with prognosis of the septic state. High expression of TFF2 was also found in skeletal muscle, liver, heart, spleen, thymus, lymph nodes and bone marrow. The exact function of TFF2 has not yet been revealed, but there is an evidence that it is connected with modulation of immune response (TFF2 deficient mice revealed significant change in expression of several genes involved in MHC class I antigen presentation), allergy and asthma (upregulation of TFF2 by diverse antigens). High levels of TFF2 in serum were also found in patients with prostate and other types of cancer (breast, colon and ovarian tumors) but its prognostic value has not yet been proved.
The RD191158100R Human Trefoil Factor 2 ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human Trefoil Factor 2.
In the BioVendor Human Trefoil Factor 2 ELISA, standards and samples are incubated in microtitrate plate wells pre-coated with polyclonal anti-human Trefoil Factor 2 antibody. After 60 minutes incubation and washing, polyclonal anti-human Trefoil Factor 2 antibody, conjugated with horseradish peroxidase (HRP) is added to the wells and incubated for 60 minutes with captured Trefoil Factor 2. Following another washing step, the remaining HRP conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of Trefoil Factor 2. A standard curve is constructed by plotting absorbance values against concentrations of Trefoil Factor 2 standards, and concentrations of unknown samples are determined using this standard curve.
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– Samson MH, Nexo E. Validation of commercial assays for measurements of trefoil factor family peptides in serum. Clin Chem Lab Med. 2011 Dec;49 (12):2057-60
– Zurek J, Kyr M, Vavrina M, Fedora M. Trefoil factor 2 expression and its significance as a predictor of severity of sepsis in children. Peptides. 2013 Apr 28;