TRAP 5 Human Assay
TRAP 5 (serum band 5 tartrate-resistant acid phosphatase, TRACP 5; EC 3.1.3.2) is a glycoprotein of 35–37 kDa. TRAP 5 belongs to the most abundant enzymes in osteoclasts. It is expressed in certain differentiated cells of the mononuclear phagocyte system, particularly osteoclasts and alveolar macrophages, where it takes an active part in bone resorption process. High blood levels of TRAP 5 are usually associated with active bone remodelling. Increased serum levels are observed during normal bone growth among healthy children. Elevated serum TRAP levels have been detected in diseases characterized by increased bone resorption; Paget’s disease of the bone, hemodialysis, primary hyperparathyroidism, metastatic malignancies involving bone resorption, multiple myeloma and bilaterally ovariectomized women. Post-menopausal women have higher levels of serum than post-menopausal women on estrogen replacement therapy. Therefore specific determination of TRAP 5 activity can be essential for clinical assessment of bone metabolism.
The RD197025000 Human TRAP 5 Assay is an ImmunoCapture Enzyme-Activity Assay for the quantitative measurement of human TRAP 5 in serum and EDTA plasma.
In the BioVendor Human TRAP 5 Assay, Calibrators, Quality Control and samples are incubated in microplate wells pre-coated with monoclonal anti-human TRAP 5 antibody. After a thorough wash, TRAP 5 bound to the antibody is allowed to react with the pNPP substrate at pH 5.5. The reaction is stopped by addition of hydroxide solution and absorbance of the resulting yellow colour product is measured spectrophotometrically at 405 nm. The absorbance is proportional to the enzymatic activity of TRAP 5. A standard curve is constructed by plotting absorbance values versus enzyme activities of recombinant TRAP 5 calibrators, and enzyme activity of unknown samples are determined (U/l) using this standard curve.
Intended use
Clinical Application
Test principle
Summary of protocol
– Halleen JM, Tiitinen SL, Ylipahkala H, Fagerlund KM, Vaananen HK. Tartrate-resistant Acid Phosphatase 5b (TRACP 5b) as a Marker of Bone Resorption. Clin Lab . 52(9-10):499-509 (2006)
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