TOTAL PROTEIN ( URINE ) Assay Kit (UP)
Determination of Total Protein in urine and cerebrospinal fluid is valuable in the diagnosis of renal and central nervous system disorders respectively. Urinary protein elevations are commonly seen in the following conditions: strenuous exercise, fever and hypothermia, nephrosis and diabetic nephropathy and urinary tract infections. Determination of total protein in cerebrospinal fluid aids in the diagnosis of such conditions as meningitis, CNS tumours and cerebral haemorrhage.
Pyrogallol red complexes with proteins in an acid environment containing molybdate ions. The resulting blue-coloured complex absorbs maximally at 600 nm. Therefore the optical density at 600 nm is directly proportional to the protein concentration of the samples.
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