Total bile acid Assay Kit (TBA)
For the in vitro quantitative determination of bile acid in serum or plasma.
The assay kit is for determination of serum total bile acids (TBA). Total bile acids are metabolized in the liver and hence serve as a marker for normal liver function. Serum total bile acids are increased in patients with acute hepatitis, chronic hepatitis, liver sclerosis and liver cancer.
The reagents of the assay kit are stable liquid formulation that allows ease of use coupled with enhanced performance characteristics. In the presence of Thio-NAD, the enzyme 3-α-hydroxysteroid dehydrogenase (3α-HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3α-HSD can convert 3-keto steroids and Thio-NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405 nm.
1. Samuel J. Levin and Morton K. Schwartz. Serum Bile Acids in Patients with Liver Disease. CLIN.CHEM. Vol. II, No. 5, 196
2. Sverre Skrede, Helge Erik Solberg, Jan Petter Biomhoff, and Egli Gjone Bile Acids Measured in Serum during Fasting as a Test for Liver Disease CLIN.CHEM.24/7, 1095-1099 (1978)
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4. Kitazima, K., and Shibata, S., Determination of serum bile acids and its use as a hepatic function test. Kawasaki