TNF-alpha Rat ELISA
TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccharides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.
Research topic
Animal studies, Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Cell culture supernatant
Sample Requirements
50 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
39.1–2500 pg/ml
Limit of Detection
11 pg/ml
Intra-assay (Within-Run)
CV = 5.0%
Inter-assay (Run-to-Run)
CV = 10.0%
Spiking Recovery
109,00%
Dilutation Linearity
114,00%
– Peric Kacarevic Z, Snajder D, Maric A, Bijelic N, Cvijanovic O, Domitrovic R, Radic R. High-fat diet induced changes in lumbar vertebra of the male rat offsprings. Acta Histochem. 2016 Aug 27;