TM-CA 72-4
The TM-CA 72-4 ELISA is an enzyme immunoassay for measurement of CA 72-4 (TAG-72) in serum and plasma.
CA 72-4 (Cancer antigen 72-4) was originally described as an antigenic determinant recognized by B 72.3, a murine monoclonal antibody raised against a membrane extract of mammary carcinoma metastases (1). CA 72-4 was identified as a 1 MDa mucine-like Glycoprotein complex termed TAG-72 (tumor associated antigen 72) (2).
The TM-CA 72-4 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal mouse antibody (Clone CC49) directed towards a unique antigenic site on a CA 72-4 molecule. An aliquot of sample containing endogenous CA 72-4 is incubated in the coated well with enzyme conjugate, which is an anti-CA 72-4 antibody (Clone B72.3) conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA 72-4 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 72-4 in the sample.