TM-CA 125
The TM-CA 125 is an enzyme immunoassay for measurement of CA 125 in serum and plasma.
The TM-CA 125 Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal [mouse] antibody directed towards a unique antigenic site of the CA 125 molecule. An aliquot of sample containing endogenous CA 125 is incubated in the coated well with enzyme conjugate, which is a monoclonal anti- CA 125 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA 125 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA 125 in the sample.
- Bast R.C. et al.: Reactivity of a monoclonal antibody with human ovarian carcinoma. J. Clin. Invest. 1981; 68:1331-1337.
- Duffy M.J.: Role of tumor markers in patients with solid cancers: A critical review. Eur. J. Intern. Med. 2007; 18(3):175-184.
- Ataseven H. et al.: Cancer antigen 125 levels in inflammatory bowel diseases. J. Clin. Lab. Anal. 2009; 23(4): 244-248.
- Powell J.L. et al.: Preoperative serum CA-125 levels in treating endometrial cancer. J. Reprod. Med. 2005; 50(8): 585-589.
- Juretzka M.M. et al.: CA125 level as a predictor of progression-free survival and overall survival in ovarian cancer patients with surgically defined disease status prior to the initiation of intraperitoneal consolidation therapy. Gynecol. Oncol. 2007; 104(1): 176-180.
- Micha J.P. et al: Clinical utility of CA-125 for maintenance therapy in the treatment of advanced stage ovarian carcinoma. Int. J. Gynecol. Cancer. 2009; 19(2): 239-241.
- Kang W.D., Choi H.S., Kim S.M.: Value of serum CA125 levels in patients with high-risk, early stage epithelial ovarian cancer. Gyneco. Oncol. 2010; 116(1): 57-60.
- Klug TL,et al.: Monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 125) associated with human epithelial ovarian carcinomas. Cancer Res. 1984; 44(3):1048-53. 9. Kaesemann H. et al.: Monoclonal antibodies in the diagnosis and follow-up of ovarian cancer. CA 125 as a tumor marker. A cooperative study of the Gynecologic Tumor Marker Group (GTMG). Klin. Wochenschr. 1986, 64(17):781-5.