Thrombomodulin (CD141) Human ELISA
Thrombomodulin (CD141) is a 557 amino acid a glycosylated transmembrane protein and is a thrombin receptor located on endothelial cell surface. This surface glycoprotein forms a complex with thrombin that activates protein C, which, in turn, degrades clotting factors V and VIII. Thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems.
Research topic
Cell surface proteins (sCD)
Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
100 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.625–20 ng/ml
Limit of Detection
< 0.38 ng/ml
– de Leeuw K, Blaauw J, Smit A, Kallenberg C, Bijl M. Vascular responsiveness in the microcirculation of patients with systemic lupus erythematosus is not impaired. Lupus. 2008 Nov;17 (11):1010-7
– de Leeuw K, Freire B, Smit AJ, Bootsma H, Kallenberg CG, Bijl M. Traditional and non-traditional risk factors contribute to the development of accelerated atherosclerosis in patients with systemic lupus erythematosus. Lupus. 2006;15 (10):675-82
– de Leeuw K, Sanders JS, Stegeman C, Smit A, Kallenberg CG, Bijl M. Accelerated atherosclerosis in patients with Wegener’s granulomatosis. Ann Rheum Dis. 2005 May;64 (5):753-9
– Jie KE, van der Putten K, Bergevoet MW, Doevendans PA, Gaillard CA, Braam B, Verhaar MC. Short- and long-term effects of erythropoietin treatment on endothelial progenitor cell levels in patients with cardiorenal syndrome. Heart. 2011 Jan;97 (1):60-53
– Skrabal CA, Choi YH, Kaminski A, Steiner M, Kundt G, Steinhoff G, Liebold A. Circulating endothelial cells demonstrate an attenuation of endothelial damage by minimizing the extracorporeal circulation. J Thorac Cardiovasc Surg. 2006 Aug;132 (2):291-6
– Yano Y, Kitagawa N, Gabazza EC, Morioka K, Urakawa H, Tanaka T, Katsuki A, Araki-Sasaki R, Hori Y, Nakatani K, Taguchi O, Sumida Y, Adachi Y. Increased plasma thrombin-activatable fibrinolysis inhibitor levels in normotensive type 2 diabetic patients with microalbuminuria. J Clin Endocrinol Metab. 2003 Feb;88 (2):736-41