TGF-beta1 Rat ELISA
TGF-beta belongs to a family of dimeric 25 kDa polypeptides that are ubiquitously distributed in tissues and synthesized by many different cells. TGF-β1 inhibits T- and B cell proliferation and inhibits maturation and activation of macrophages. It furthermore inhibits activity of natural killer cells and lymphokine activated killer cells and blocks production of cytokines.
Research topic
Animal studies, Cytokines and chemokines and related molecules
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Citrate, Cell culture supernatant
Sample Requirements
100 µl (pretreated)
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
31.25–2000 pg/ml
Limit of Detection
12 pg/ml
Intra-assay (Within-Run)
CV = 6.8%
Inter-assay (Run-to-Run)
CV = 6.8%
– Allam MM. Insulin Like Growth Factor -1(IGF-1) Promotes Angiogenesis and Reverses Ischemia Reperfusion Induced Acute Kidney Injury in Rats: Role of VEGF and TGF-β1. American Journal of Biomedical. May 2016;