Tetanus Toxoid IgG ELISA
The IMMUNOLAB Tetanus Toxoid IgG Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgG antibodies against Tetanus Toxoid in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service of IMMUNOLAB.
Tetanus is a disease caused by the toxin from Clostridium tetani. Through better hygienic conditions and a wide prophylaxis by vaccination, the disease rate could be decreased worldwide. Nevertheless every year 400,000 – 800,000 persons die by this infection. The majority of these persons live in under-developed countries. The protection through vaccination is very rare in older persons, because Tetanus antitoxin levels decline with age. The immunity against Tetanus has a vital significance for a lot of actions in business and free time. Sufficient protection is achieved by vaccination and following booster injections. Protection begins at a level of 0.1 IU/mL of anti-Tetanus Toxoid. There is only a very low vaccination risk. Nevertheless it is advisable to detect the immunity with a qualified test before boostering. By this way it is possible to prevent the patient of side effects like local swelling, pain and fever. Failure to respond to one or more antigens can sometimes be observed in patients with normal or high levels of all immunoglobulins, and in patients with isolated immunodeficiencies. Thus, normal immunoglobulin concentrations do not exclude antibody deficiency, and response to antigenic stimulation should be tested. If antibody determinations are performed over an extended period of time after priming and boostering, abnormalities in the rate of decline of cellular interactions as well as disorders in peak titers.
The IMMUNOLAB Tetanus Toxoid IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). Tetanus antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgG antibodies of the serum and the immobilized Tetanus Toxoid antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgG peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgG antibodies is directly proportional to the intensity of the color.
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