Testosterone ELISA
The Calbiotech, Inc. (CBI) Testosterone ELISA Kit is intended for the quantitative measurement of Testosterone in human serum or plasma.
Testosterone (17b-hydroxyandrost-4-ene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the b position at C-17. This steroid hormone has a molecular weight of 288.4. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. The Testosterone EIA kits are designed for the measurement of total Testosterone in human serum.
The Calbiotech, Inc. Testosterone test kit is a solid phase competitive ELISA. The samples, working Testosterone-HRP Conjugate and anti-Testosterone-biotin solution are added to the wells coated with streptavidin. Testosterone in the patient’s serum competes with the Testosterone enzyme (HRP) conjugate for binding sites. Unbound Testosterone and testosterone enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Testosterone in the samples. A standard curve is prepared relating color intensity to the concentration of the Testosterone.
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