Soluble Receptor of Advanced Glycation End-Products (sRAGE) Human ELISA
The receptor for advanced glycation end products (RAGE) is a multiligand member of the immunoglobulin superfamily of cell surface molecules. It mediates interactions of advanced glycation end products (AGE), the products of nonenzymatic glycoxidation of proteins/lipids that accumulate in the plasma and tissues of patients with diabetes.
The RD191116200R Human sRAGE ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human sRAGE (soluble receptor for advanced glycation end products).
In the Biovendor Human sRAGE ELISA, standards, quality controls and samples are incubated in microplate wells pre-coated with polyclonal anti-human sRAGE antibody. After 120 minutes incubation and washing, biotin labelled polyclonal anti-human sRAGE antibody is added and incubated with captured sRAGE for 60 minutes. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of sRAGE. A standard curve is constructed by plotting absorbance values versus sRAGE concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
Intended use
Clinical Application
Test principle
Summary of protocol
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