Sialic Acid Assay Kit (SA)
For the quantitative determination of human Sialic acid in serum by enzymatic colorimetric assay. For in vitro diagnostic use only.
Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone.Increased SA concentrations have been observed in several diseases, e.g., tumors, myocardial infarction, diabetes, inflammatory disorders, and alcoholism.
Sialic in specimen is converted to N-Acetylneuraminic acid by neuraminidase;then N-Acetylneuraminic acid is converted to N-acetylmannosamine and Pyruvic acid by NANA-aldolase. The pyruvate formed reacts with NADH in the presence of LDH to form Lactate and NAD. The corresponding decrease in absorbance at 340nm is proportional to the sialic concentration.
1. Sugahara, K., et al., Enzymatic assay of serum sialic acid. Clin. Chim. Acta, 108, 493-498 (1980).
2. Hannu alho,et al,Serum sialic acid in a random sample of the general population.General Clinical Chemistry,45,No.10,1842-1849(1999).