Serotonin
Enzyme Immunoassay for the fast and quantitative determination of Serotonin in urine, plasma and serum
Characteristics
Very fast assay with simple sample preparation
Total incubation time approx. 1.5 hours
No interference by medical drugs
Break apart wells for flexible assay runs
Wide standard range, convenient measurement of pathological samples without predilution
Serotonin (5-Hydroxytryptamine), a biogenic amine, is a product of the tryptophan metabolism. It is a well evaluated neurotransmitter of the central nervous system and can be found in high concentrations in the chromaffine cells of the intestinal mucosa, in the platelets and the serotonergic neurones of the brain.
Central-serotonergic neurones influence physiological functions such as sleep and the hormonal and cardio-vascular regulation. Increased serum levels can be found with malignant carcinoid, endogenous depression and schizophrenia.
The assay kit provides materials for the quantitative measurement of derivated serotonin (5-Hydroxytryptamine) in serum, plasma and urine. The derivation is performed during the preparation of the samples. By using the acylation reagent the serotonin is quantitatively derivated into N-acylserotonin.
The competitive Serotonin ELISA kit uses the microtitre plate format. Serotonin is bound to the solid phase of the microtiter plate. Acylated serotonin and solid phase bound serotonin compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase serotonin is detected by antirabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase serotonin is inversely proportional to the serotonin concentration of the sample.
- Kema, P.; de Vries, E.; Muskiet, F. (2000): Clinical chemistry of serotonin and metabolites
Journal of Chromatography B, 747 33–48 - Lechin, F.; van der Dijs, B.; Lechin, A. (2005): Circulating Serotonin,Catecholamines, and CentralNervous System Circuitry Relatedto Some Cardiorespiratory,Vascular, and HematologicalDisorders The Journal of Applied Research Vol. 5, No. 4
- Spaeth, M. (2006): Fibromyalgia Syndrome:The Role of Neurochemicals Primary Psychiatry. 13(9):72-75
- Spivak, B.; Vered, Y.; Graff, E.;. et al. (1999): Low Platelet-Poor Plasma Concentrations of Serotoninin Patients with Combat-Related Posttraumatic Stress Disorder BIOL PSYCHIATRY. 45:840–845
- Ernberg, M.; Lundeberg, T.; Kopp, S. (2000): Pain and allodynia/hyperalgesia induced by intramuscular injection of serotonin in patients with fibromyalgia and healthy individuals
Pain 85 31±39 - Alvarez, J.; Gluck, N.; Fallet, A.; et al. (1999): Plasma serotonin level after 1 day of fluoxetine treatment: a biological predictor for antidepressant response? Psychopharmacology 143 : 97.101
- Mück-Seler, D.; Pivac, N.; Jakovljevic, M.; et al. (1999): Platelet Serotonin, Plasma Cortisol, and Dexamethasone Suppression Test in Schizophrenic Patients BIOL PSYCHIATRY 45:1433–1439
- Vikenes, K.; Farstad, M.; Nordrehaug, J. (1999): Serotonin Is Associated with Coronary Artery Disease and Cardiac Events Circulation August 3, 1999; 483-489
- Dayan, P.; Huys, Q.. (2008): Serotonin, Inhibition, and Negative Mood PLoS Computational Biology February 2008 | Volume 4 | Issue 2 | e4
- Leboyer, M.; Philippe, A.; Bouvard, M.; et al. (1999): Whole Blood Serotonin and Plasma Beta-Endorphin in Autistic Probands and Their First-Degree Relatives BIOL PSYCHIATRY 1999;45:158–163