Resistin Rat ELISA
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Rat resistin contains 114 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in rat blood as a dimeric protein consisting of two 96 amino acid polypeptides, which are disulfide-linked. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs (TZDs). On the other hand, several studies demonstrated reduced resistin expression in adipose tissue of obese mice and increased levels in leptin deficient ob/ob mice and Zucker diabetic fatty rats in response to TZDs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.
Areas of investigation: Insulin resistance, Obesity
Features
- It is intended for research use only
- The total assay time is less than 4 hours
- The kit measures total serum (homodimeric) resistin
- Assay format is 96 wells
- Quality Controls are recombinant rat resistin based
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Animal studies, Diabetology – Other Relevant Products, Energy metabolism and body weight regulation
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum
Sample Requirements
5 µl/well
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.25–20 ng/ml
Limit of Detection
Analytical Limit of Detection is calculated from the real Resistin values in wells and is 0.05ng/ml
Intra-assay (Within-Run)
n = 8; CV = 5.1%
Inter-assay (Run-to-Run)
n = 5; CV = 7.1%
Spiking Recovery
92,10%
Dilutation Linearity
112,70%
Crossreactivity
bovine Non-detectable cat Non-detectable dog Non-detectable hamster Non-detectable horse Non-detectable goat Yes monkey Non-detectable mouse Non-detectable pig Non-detectable rabbit Non-detectable rat Non-detectable sheep Non-detectable human Non-detectable chicken Not tested
– Aborehab NM, El Bishbishy MH, Waly NE. Resistin mediates tomato and broccoli extract effects on glucose homeostasis in high fat diet-induced obesity in rats. BMC Complement Altern Med. 2016;16 (1):225
– Alp A, Buyukbas S, Harun Alp, Gergerlioglu HS, Oz M, Basarali MK, Kiyici A. Effects of exercise and caffeic acid phenethyl ester after chronic exercise rat model. J Sports Sci Med . 2011;10:649-654
– Bonzon-Kulichenko E, Fernandez-Agullo T, Molto E, Serrano R, Fernandez A, Ros M, Carrascosa JM, Arribas C, Martinez C, Andres A, Gallardo N. Regulation of insulin-stimulated glucose uptake in rat white adipose tissue upon chronic central leptin infusion: effects on adiposity. Endocrinology. 2011 Apr;152 (4):1366-77
– Castillero E, Martin AI, Nieto-Bona MP, Fernandez-Galaz C, Lopez-Menduina M, Villanua MA, Lopez-Calderon A. Fenofibrate administration to arthritic rats increases adiponectin and leptin and prevents oxidative muscle wasting. Endocr Connect . 2012;01, 1–12
– Chabrolle C, Jeanpierre E, Tosca L, Rame C, Dupont J. Effects of high levels of glucose on the steroidogenesis and the expression of adiponectin receptors in rat ovarian cells. Reprod Biol Endocrinol. 2008;6:11
– Enas N. Morgan and Amal M H. Mackawy. The Association of Resistin Polymorphism (3’UTR+62GA) with Insulin Resistance and Hypertension at High Fat Diet Induced Type2 Diabetes in Rats: Experimental Study. Journal of American Science. 2011;7(10)
– Escriva F, Gavete ML, Fermin Y, Perez C, Gallardo N, Alvarez C, Andres A, Ros M, Carrascosa JM. Effect of age and moderate food restriction on insulin sensitivity in Wistar rats: role of adiposity. J Endocrinol. 2007 Jul;194 (1):131-41
– Fernandez CM, Molto E, Gallardo N, del Arco A, Martinez C, Andres A, Ros M, Carrascosa JM, Arribas C. The expression of rat resistin isoforms is differentially regulated in visceral adipose tissues: effects of aging and food restriction. Metabolism. 2009 Feb;58 (2):204-11
– Manneras L, Fazliana M, Wan Nazaimoon WM, Lonn M, Gu HF, Ostenson CG, Stener-Victorin E. Beneficial metabolic effects of the Malaysian herb Labisia pumila var. alata in a rat model of polycystic ovary syndrome. J Ethnopharmacol. 2010 Feb 3;127 (2):346-51
– Mansor F. Studies on Labisia pumila var. alata extract with phytoestrogenic effects: impact on biological activities and gene expression. Stockholm 2010.
– Sassek M, Pruszynska-Oszmalek E, Kolodziejski PA, Szczepankiewicz D, Kaczmarek P, Wieloch M, Kurto K, Nogowski L, Nowak KW, Strowski MZ, Mackowiak P. Resistin is produced by rat pancreatic islets and regulates insulin and glucagon in vitro secretion. Islets. 2016 Oct 31;:1-9
– Stroubini T, Perelas A, Liapi C, Perrea D, Dontas I, Tzavara Ch, Galanopoulou P. Serum adiponectin and resistin in rats under three isocaloric diets: The effect of sibutramine. Cytokine. 2009 May;46 (2):171-5
– Tatsumi K, Sasaki H, Fujita A, Doi A, Kanaya Y, Furuta H, Nishi M, Tsuno T, Taniguchi H, Nanjo K. Effect of anti-oxidants, Ricetrienol and α-tocopherol, on adipocytokine abnormalities and fatty liver in Otsuka Long-Evans Tokushima Fatty diabetic rats. J Diabetes Investig. June 2011;2:186–192