Prolactin ELISA
The Calbiotech, Inc. (CBI) Prolactin ELISA kit is used for the quantitative measurement of prolactin in human serum.
Human prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland in both men and woman. Women normally have slightly higher basal prolactin levels than men. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications. Hyperprolactinemia inhibits hypogonadism in men and women with accompanying low FSH and LH levels.
The Prolactin ELISA kit is a solid phase sandwich ELISA assay method, based on a streptavidin-biotin principle. The standards, samples and a reagent mixture of Anti-Prolactin Enzyme and Biotin conjugates are added into the wells, coated with Streptavidin. Prolactin in the patient’s serum forms a sandwich between two highly specific Prolactin antibodies, labeled with Biotin and HRP. Simultaneously, the biotinylated antibody is immobilized onto the well through a high affinity Streptavidin-Biotin interaction. Unbound protein and excess biotin/enzyme conjugated reagent are washed off by wash buffer. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of Prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the Prolactin.
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