Proguanylin Human ELISA
Proguanylin, the 116-amino acid prohormone, is a bioactive form of human guanylin that acts on intestinal guanylate cyclase, thereby regulating intestinal fluid and elektrolyte transport through the second messenger, cyclic GMP (cGMP). The cGMP increase inhibits salt absorption and stimulates chloride secretion into the gut. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to diarrhea and dehydratation characteristic of enterotoxin activity.
Proguanylin is found in circulation and plays an endocrine role by regulating the function of tissues such as the kidney and liver. Proguanylin is a significant marker in renal insufficiency. Plasma levels of proguanylin increase in patients with chronic renal failure who were undergoing hemodialysis.
Studies have shown, that serum levels of Proguanylin has rise in patients with Cohn syndrome therefore it could be used as a novel marker in diagnostic and therapy of cardiac failure.
Features
- It is intended for research use only
- The total assay time is less than 3 hours
- The kit measures total proguanylin in serum, plasma
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Others, Renal disease
Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Plasma (EDTA, citrate, heparin)
Sample Requirements
20 µl/well
Storage/Expiration
Store the complete kit at 2 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.31–10 ng/ml
Limit of Detection
0.06 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 5.2 %
Inter-assay (Run-to-Run)
n = 6; CV = 4.1 %
Spiking Recovery
95.2 %
Dilutation Linearity
97,10%
Crossreactivity
bovine Non-detectable cat Non-detectable dog Non-detectable goat Non-detectable hamster Non-detectable horse Non-detectable monkey Non-detectable mouse Non-detectable pig Non-detectable rabbit Non-detectable rat Non-detectable sheep Non-detectable chicken Not tested human Yes
– Kaar G, Dieplinger B, Gabriel C, Haltmayer M, Mueller T. Proguanylin and prouroguanylin–assay evaluation and clinical analyte characterization. Clin Chim Acta. 2011 Nov 20;412 (23-24):2277-83
– Solichova P, Stejskal D, Proskova J. Guanylins – Agents with natriuretic effect. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub . Jul;150(1):85-7 (2006)
– Stejskal D, Solichova P, Lacnak B, Vaclavik J, Hanulova Z, Humenanska V, Sprongl L, Karpisek M. Proguanylin: development, analytical characterization, and clinical testing of a new ELISA. Gen Physiol Biophys . Mar;26(1):62-5 (2007)