Potassium Assay Kit (K)
For the in vitro quantitative determination of potassium in serum or plasma.
Potassium Assay has an outstanding linearity range from 2.0 mmol/L – 8.0 mmol/L. Potassium Assay offers excellent precision CV% of <2%. A comparison study showed that the assay has good correlation with existing ISE method’s with a correlation coefficient value of 0.98, slope of 1.07 and y intercept of -0.30.
Potassium is determined enzymatically via potassiumdependant pyruvate kinase activity using phosphoenolpyruvate as substrate. The pyruvate formed reacts with NADH in the presence of LDH to form Lactate and NAD. The corresponding decrease in absorbance at 340nm is proportional to the potassium concentration.
PEP + ADP → Pyruvate + ATP
LDH
Pyruvate + NADH + H+ → lactate + NAD+
- Berry, M. N. et al., (1988) Clin. Chem.35:817
- Tietz, N. W.(1986). textbook of clinical Chemistry, p.1841. W. B. Saundres Company, Philadelphia.