PLGF
The PLGF ELISA is an enzyme immunoassay for measurement of human placenta growth factor (PLGF) in serum.
The PLGF ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a monoclonal antibody directed towards a unique antigenic site of the PLGF molecule. An aliquot of specimen sample containing endogenous PLGF is incubated in the coated well. After a washing step a biotin-linked polyclonal antibody specific for PLGF is added to the wells. Following a wash to remove any unbound antibody a streptavidin HRP enzyme complex is added to the wells. After incubation the unbound enzyme complex is washed off. The amount of bound peroxidase is proportional to the concentration of PLGF in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of PLGF in the specimen sample
- Levine RJ, Thadhani R, Qian C, Lam C, Lim KH, Yu KF, Blink AL, Sachs BP, Epstein FH, Sibai BM, Sukhatme VP, Karumanchi SA. Urinary placental growth factor and risk of preeclampsia. JAMA. 2005 Jan 5;293(1):77-85.
- Levine RJ, Maynard SE, Qian C, Lim KH, England LJ, Yu KF, Schisterman EF, Thadhani R, Sachs BP, Epstein FH, Sibai BM, Sukhatme VP, Karumanchi SA. Circulating angiogenic factors and the risk of preeclampsia. N Engl J Med. 2004 Feb 12;350(7):672-83. Epub 2004 Feb 5.