PAPP-A
The PAPP-A ELISA is an enzyme immunoassay for measurement of Pregnancy associated plasma protein A (PAPP-A) in serum and plasma
Test Principle
The PAPP-A ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a polyclonal anti PAPP-A antibody. An aliquot of sample containing endogenous PAPP-A is incubated in the coated well with assay buffer. After incubation the unbound material is washed off. In the second incubation step a sandwich complex is formed with a polyclonal anti PAPP-A antibody peroxidase conjugate. Having added the substrate solution, the intensity of color developed is proportional to the concentration of PAPP-A in the sample.
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References
- Spencer K., Scouter V., Tul N., Snijders R., Nicolaides K.H. Ultrasound Obset Gynecol. 1999; 13:1-7
- Sancken U. Deutsches Ärzteblatt 97 3.März 2000, Heft 9:A532-A537
- Bayes-Genis A., Conover C.A., Overgaard M.T., et al. N. Engl. J. Med., Vol.345, No.14, 2001: 1022-1029
- Schaeffer H.-J., Sancken U., Eiben B., Opper C., Jung A. Fetal Diagn Ther. 2001; 16: 437-464
- Qiu-Ping Q., Christiansen M., Oxvig C., Pettersson K., Sottrup-Jensen L., Koch C., Norgaard-Pedersen B. Clinical Chemistry 43:12, 1997; 2323-2332
- Wald NJ, Rodeck C, Hackshaw AK, Walters J, Chitty L, Mackinson AM. Health Technol Assess. 2003; 7(11). First and second trimester antenatal screening for Down´s Syndrom: the results of the Serum, Urine and Ultrasound Screening Study (SURUSS).
- Bindra R, Heath V, Liao A, Spencer K, Nicolaides KH. Ultrasound Obstet Gynecol. 2002 Sep;20(3): 219-25. One-stop clinic for assessment of risk for trisomy 21 at 11-14 weeks: a prospective study of 15 030 pregnancies.
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