NSE
Measurement of hNSE concentration in human serum. NSE ELISA kit is intended for laboratory use only.
Test Principle
The NSE ELISA test is based on simultaneous binding of human Neuron Specific Enolase by two monoclonal antibodies, one immobilized on microwell plates and the other conjugates with horseradish peroxidase (HPR). After incubation the bound/free separation is performed by a simple solid-phase washing, then the TMB-Substrate solution (TMB) is added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbancies are determinated. The colour intensity is proportional to the hNSE concentration in the sample. hNSE concentration in the sample is calculated based on a Calibration curve.
Order Enquiry
References
- Sorensen K, Brodbeck U, Paus E, Norgaard-Pedersen B. An enzyme antigen immunoassay for the determination of neuron-specific enolase in serum samples. Clin Chim Acta. 1988 Jul 29;175(3):337-43.
- Drivsholm L, Osterlind K, Cooper EH, Purves DA. Neuron-specific enolase (NSE) in serum. Comparison of monoclonal versus polyclonal assay based on 392 blood samples. Int J Biol Markers. 1995 Jan-Mar;10(1):1-4.
- Karnak D, Beder S, Kayacan O, Ibis E, Oflaz G. Neuron-specific enolase and lung cancer. Am J Clin Oncol. 2005 Dec;28(6):586-90.
- Berger RP, Dulani T, Adelson PD, Leventhal JM, Richichi R, Kochanek PM. Identification of inflicted traumatic brain injury in well-appearing infants using serum and cerebrospinal markers: a possible screening tool. Pediatrics. 2006 Feb;117(2):325-32.
- Ghayumi SM, Mehrabi S, Doroudchi M, Ghaderi A Diagnostic value of tumor markers for differentiating malignant and benign pleural effusions of Iranian patients. Pathol Oncol Res. 2005;11(4):236-41. Epub 2005 Dec 31.
- Sawauchi S, Taya K, Murakami S, Ishi T, Ohtsuka T, Kato N, Kaku S, Tanaka T, Morooka S, Yuhki K, Urashima M, Abe T. Serum S-100B protein and neuron-specific enolase after traumatic brain injury. No Shinkei Geka. 2005 Nov;33(11):1073-80
- Ramont L, Thoannes H, Volondat A, Chastang F, Millet MC, Maquart FX. Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice. Clin Chem Lab Med. 2005;43(11):1215-7.
Documents