Murine IL-1α ELISA Kit
The mIL-1α ELISA is an enzyme-linked immunosorbent assay for quantitative detection of murine Interleukin1α in cell culture supernatants, murine serum, plasma or other body fluids.
The interleukin-1 (IL-1) species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1α and IL-1β, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.
An anti-mIL-1α monoclonal coating antibody is adsorbed onto microwells.
mIL-1α present in the sample or standard binds to antibodies adsorbed to the microwells; a biotin-conjugated monoclonal anti-mIL-1α antibody is added and binds to mIL-1α captured by the first antibody.
Following incubation unbound biotin conjugated anti-mIL1α is removed during a wash step. Streptavidin-HRP is added and binds to the biotin conjugated anti-mIL-1α. Following incubation unbound Streptavidin-HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells.
A coloured product is formed in proportion to the amount of mIL-1α present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven mIL-1α standard dilutions and mIL-1α sample concentration determined.