MTB IgG
Enzyme ImmunoAssay (ELISA) for the qualitative and/or semi quantitative determination of IgG antibodies to Mycobacterium tuberculosis.
Mycobacterium tuberculosis (MTB) is a fastidious, slowlygrowing, strictly aerobic bacterium with a complex cell wall composed of peptide-glycans and many complex long-chain lipids.
Tuberculosis remains one of the most common and deadly diseases throughout the world. In the past 10 years there has been a resurgence of tuberculosis in old-world countries, also due to new infections (HIV) and immigration.
In the diagnosis of Tuberculosis and in the follow-up of infected patients, ELISA for antibodies may be useful to provide information on the immunological status of the patient, in addition to Nucleic Acid Tests (or NATs) able to determine the presence of the bacterium itself.
Microplates are coated with a chimeric recombinant antigen bearing the most immunogenic epitopes of MTB.
The solid phase is first treated with the diluted sample and anti MTB IgG are captured, if present, by the antigens coated on the microplate.
After washing out all the other components of the sample, in the 2nd incubation bound anti MTB IgG antibodies are detected by the addition of polyclonal specific anti hIgG antibodies, labeled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti MTB IgG antibodies present in the sample.
IgG in the sample may therefore be semi quantitated in arbU/ml by means of its S/Co value and a calibration curve.
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