Monocyte Chemotactic Protein-1 Human ELISA
Monocyte chemotactic protein-1 (MCP-1) is a member of the C-C family of chemokines. Chemokines are mediators that share the capacity to induce directional migration of leukocytes. MCP-1 is a chemoattractant for many cell types such as monocytes, neutrophils, hepatic stellate cells, granulocytes and T lymphocytes. MCP-1 is believed to play a role in a variety of pathological conditions, including inflammatory and immune reactions, atherosclerosis and neoplasia. Monocyte Chemotactic Protein-1 (MCP-1) is also known as Monocyte Chemotactic Activating Factor (MCAF) or CCL2.
Research topic
Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation
Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Cell culture supernatant
Sample Requirements
20 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
15.6–1000 pg/ml
Limit of Detection
2.3 pg/ml
Intra-assay (Within-Run)
CV = 4.7%
Inter-assay (Run-to-Run)
CV = 8.7%
Spiking Recovery
92,00%
Dilutation Linearity
105,00%
– Fort-Gallifa I, García-Heredia A, Hernández-Aguilera A, Simó JM, Sepúlveda J, Martín-Paredero V, Camps J, Joven J. Biochemical indices of oxidative stress and inflammation in the evaluation of peripheral artery disease. Free Radical Biology and Medic. July 2016;