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Lipase Assay Kit (LPS)

For the quantitative in vitro determination of Lipase in human serum and plasma.

Regulatery Status: CE

Product Catalog Number Packaging Method/Format
Product Catalog No: Kit (80 Ml) Pack Size: GSLPS Pack Size: R1: 1×60 ml
R2: 1×20 ml 		Pack Size: Methoxyresorufin Substrate
Product Catalog No: Kit (80 Ml) Pack Size: GBLPS Pack Size: R1: 1×60 ml
R2: 1×20 ml
Product Catalog No: Kit (60 Ml) Pack Size: GHLPS Pack Size: R1: 1×45 ml
R2: 1×15 ml
Product Catalog No: Kit (60 Ml) Pack Size: GTLPS Pack Size: R1: 1×45 ml
R2: 1×15 ml
Product Catalog No: Kit (80 Ml) Pack Size: GXLPS Pack Size: R1: 1×60 ml
R2: 1×20 ml
Product Catalog No: Kit (135.6 Ml) Pack Size: GDLPS Pack Size: R1: 24×4.2 ml
R2: 12×2.9 ml
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Product Features

A lipase test system is a device intended to measure the activity of the enzyme lipase in serum and plasma. Lipase measurements are used in the diagnosis and treatment of diseases of the pancreas such as acute pancreatitis and obstruction of the pancreatic duct.

Assay Principle

The chromogenic Lipase substrate 1,2-o-dilaurylracglycero-3-glutaric acid-(6ı-methylre-sorufin) ester is cleaved by the catalytic action of Lipase to form 1,2-odilauryl racglycerol and an unstable intermediate, glutaric acid-(6-methyl resorufin)ester.This decomposes spontaneously in alkaline solution to form glutaric acid and methylresorufin. The lipase activity in the speci-men is proportional to the production of methy-lresorufin in the reaction and can be determined photometrically.

1,2-O-dilauryl-rac-glycero-3-glutar acid-(6 methylresorufin) ester → 1,2-O-dilauryl-rac-glycerol + glutaric acid-(6 methylresorufin) ester → glutaric acid+methylresorufin

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References
  • Greiling H, Gressner AM, eds. Lehrbuch d-er Klinischen Chemie und Pathobiochemie, 3rd ed. Stuttgart/NewYork: Schattauer V-erlag, 1995.
  • Keller H, ed. Klinisch-chemische Labordia-gnostik für die Praxis, 2nd ed.Stuttgart/New York: Georg Thieme Verlag, 1991:354-361.
  • Kazmierczak S, Catrou P, Van Lente F. Diagnostic accuracy of pancreatic enzymes evaluated by use of multivariate data ana-lysis. Clin Chem 1993; 39: 1960-1965.
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