Leptin Receptor Human ELISA
Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16 kDa protein consisting of 146 amino acid residues.) OB R was found to be a member of the class I cytokine receptor family with a large extracellular domain comprising 816 amino acid residues. Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Alternate splicing from a single gene derives the six isoforms of the Leptin receptor. The soluble form of the Leptin receptor, OB R contains no intracellular motifs or transmembrane residues, thus it consists entirely of the extracellular ligand binding domain of the receptor. Long forms of OB-R transcripts were reported to be expressed predominantly in regions of the hypothalamus which provides evidence that Leptin receptor is important in body weight regulation. Expression of short forms of OB-R transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia.
The RD194002100 Human Leptin Receptor ELISA is a sandwich enzyme immunoassay for the quantitative measurement of human leptin receptor.
In the BioVendor Human Leptin Receptor ELISA, Standards, Quality Controls and samples are incubated in microplate wells pre-coated with monoclonal anti-human leptin receptor antibody. After 60 minutes incubation and washing, monoclonal anti-human leptin receptor antibody, conjugated with horseradish peroxidase (HRP) is added to the wells and incubated for 60 minutes with captured leptin receptor. Following another washing step, the remaining HRP conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of leptin receptor. A standard curve is constructed by plotting absorbance values against concentrations of Standards, and concentrations of unknown samples are determined using this standard curve.
Intended use
Clinical Application
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Summary of protocol
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