Legionella pneumophila Ag (urine)
This Legionella Urinary Antigen test is intended for the detection of Legionella pneumophilia antigen in human urine.
Test Principle
This assay is a double antibody (sandwich) ELISA using an anti-Legionella pneumophilia antibody to capture the antigen from the urine. A second antibody, conjugated to horseradish peroxidase (HRP), is then added which binds to the complex. This reaction is visualized by the addition of the chromogen tetramethylbenzidine (TMB). The resulting blue color development indicates the presence of Legionella pneumophilia antigens being bound by the anti- Legionella pneumophilia antibodies. Addition of a stop solution ends the reaction and turns the blue color to yellow.
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References
- Fraser D.W., Tsai T.R., Orenstein W., Parkin W.E., Beecham H.J., Sharrar R.G., Harris J., Mallison G.F., Martin S.M., McDade J.E., Shepherd C.C., Brachman P.S., and the Field Investigation Team. 1977. Legionnaires’ Disease: Description of an epidemic of pneumonia. N. Engl. J. Med. 29:1189-1197.
- Stout J.E., Yu V.L., 1977. Legionellosis. N. Engl. J. Med. 337:682-687.
- Kohler R.B., Wilde III C., Johnson W., Joly J., Wheat L.J., Baker R., and Misfeldt M. 1988. Immunologic diversity among serogroup 1 Legionella pneumophilia urinary antigens demonstrated by monoclonal antibody enzyme-linked immunosorbent assays. J. Clin. Microbiol. 26:2059-2063.
- Kohler R.B., Winn W. C. Jr., and Wheat L.J. 1984. Onset and duration of urinary antigen excretion in Legionnaires’ Disease. J. Clin. Microbiol. 20:605-607.
- Berdal B.P., Farshy C.E., and Freeley J.C. 1979. Detection of Legionella pneumophilia antigen in urine by enzyme-linked immunospecific assay. J. Clin. Microbiol. 9:575-578.
- Tilton R.B. 1979. Legionnaires’ Disease antigen detected by enzyme-linked immunosorbent assay. Ann. Itern. Med. 90:697-698.
- Kohler R.B., Zimmerman S.E., Wilson E., Allen S.D., Edelstein P.H., Wheat L.J., and White A. 1981. Rapid radioimmunoassay diagnosis of Legionnaires’ Disease. Ann. Itern. Med. 94:601-605.
- Bibb W.F., Arrow P.M., Thacker L., and McKinney R.M. 1984. Detection of soluble Legionella pneumophilia antigens in serum and urine specimens by enzyme-linked immunosorbent assay with monoclonal and polyclonal antibodies. J. Clin. Microbiol. 20:478-482.
- Tang P.W., and Toma S. 1986. Broad spectrum enzyme-linked immunosorbent assay for detection of Legionella soluble antigens. J. Clin. Microbiol. 24:556-558.
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