Lactate Assay Kit (LAC)
For the quantitative in vitro determination of Lactate in serum or plasma.
In muscle cells, lactate concentration showed metabolic acidosis, which may be lactic acidosis. Tissue hypoxia and strenuous activities will increase in serum lactate concentration. Tissue hypoxia caused by respiratory failure or hypoperfusion, which lactic acidosisi can threaten life. Dehydration can cause the reduction of muscle cells oxygen transfer. Lactic acidosis may often accompany with diabetes ketoacidosis. Tissue oxygen consumption increased, as seen in sepsis and malignant tumors can also cause lactic acidosis. The severity of lactic acidosis can help reveal the potential severity of the sexual disease.
Lactate oxidase is oxidation with lactate, produce pyruvate and hydrogen peroxide, hydrogen peroxide reacts with a 4 – amino antipyrine, TOOS, produces purple reaction product, this product has a maximum absorption peak at 546nm, the absorption intensity is proportionalto the lactic acid content in the samples.
LOX
Lactate+ O2 → pyruvate + H2O2
POD
H2O2 + 4-ALDEHYDOANTIPYRINE → purple product + H2O + TOOS
- HenryRJ. Clinical Chemistry :Principlesand Technics.Harper and Row New York 1974.
- S.LACimura, S.Iyama, Y.Yamagychi S.hayashi, R.Fush-imimi and Amino .Ann.LACin.Biochem(1977), 34:384-38 388.