JE (Japanese Encephalitis) IgM Antibody Capture
The JE IgM ELISA test for exposure to Japanese Encephalitis Virus (JEV) is an ELISA assay system for measurement of IgM antibodies in human serum to JEV derived recombinant antigen (JERA) (1-4). It is not intended to screen blood or blood components.
Exposure to JEV causes a disease with a number of symptoms including encephalitis (5-8). The JE IgM ELISA employs a recombinant antigen called JERA, which can be used as a rapid serological marker for JEV infection. The JERA protein is a recombinant antigen, which consists of a stretch of peptides from different parts of the JE.
The JE IgM ELISA consists of one enzymatically amplified “two-step” sandwich-type immunoassay. In this assay, JE Detect Low Control (represents non-reactive serum), JE Detect IgM High Control (represents reactive serum), and unknown serum samples are diluted with Sample Dilution Buffer for JE Detect IgM, then incubated in microtitration wells which have been coated with anti-human IgM antibodies. This is followed by incubation with both JEV derived recombinant antigen (JERA) and Normal Cell Antigen (NCA) separately. After incubation and washing, the wells are treated with a JERA-specific antibody labeled with the enzyme horseradish peroxidase (HRP). After a third incubation and washing step, the wells are incubated with the tetramethylbenzidine (TMB) substrate. An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. Above a certain threshold, the ratio of the absorbencies of the JERA and the control wells accurately determines whether antibodies to JEV are present.
- Martin, D.A., Muth, D.A., Brown, T., Johnson, A.J., Karabatsos,R, Roehrig, J.T. 2000. Standardization of Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assays for Routine Diagnosis of Arboviral Infections. J. Clin. Microbiol. 38(5):1823-1826.
- Cardosa MJ, Wang SM, Sum MS, Tio PH. Antibodies against prM protein distinguish between previous infection with dengue and Japanese encephalitis viruses. BMC Microbiol. 2002 May 5;2(1):9
- Pandey B, Yamamoto A, Morita K, Kurosawa Y, Rai S, Adhikari S, Kandel P, Kurane I. Serodiagnosis of Japanese encephalitis among Nepalese patients by the particle agglutination assay. Epidemiol Infect. 2003 Oct;131(2):881-5.
- Thakare JP, Gore MM, Risbud AR, Banerjee K, Ghosh SN. ion of virus specific IgG subclasses in Japanese encephalitis patients. Indian J Med Res. 1991 Sep;93:271-6.
- Lowry PW, Truong DH, Hinh LD, Ladinsky JL, Karabatsos N, Cropp CB, Martin D, and Gubler DJ. Japanese encephalitis among hospitalized pediactric and adult patients with acute encephalitis syndrome in Hanoi, Vietnam 1995. Am. J. Trop. Med. Hyg, 1998;58(3):324-329.
- Tsai TF. Factors in the changing epidemiology of Japanese encephalitis and West Nile fever. In: Saluzzo JF ed., Factors in the Emergence of Arboviral Diseases. Amsterdam: Elsevier, 1997;179-189.
- Tsai TF. Japanese encephalitis. In: Feigin RD and Cherry JD (eds.), Textbook of Pediatric Infectious Diseases, 4th edition, Philadelphia: W.B. Saunders, 1997;1993-2001.
- Rosen L. The natural history of Japanese encephalitis. Annu. Rev. Microbiol., 1986;40:395-414.