Japanese Encephalitis IgG ELISA
The JE IgG test for exposure to Japanese Encephalitis Virus (JEV) is an ELISA assay system for the detection of antibodies in human serum to JEV derived recombinant antigen (JERA) (1-4).
Exposure to JEV causes a disease with a number of symptoms including encephalitis (5-8). The JE IgG assay employs a recombinant antigen called JERA, which can be used as a rapid serological marker for JEV infection. The JERA protein is a recombinant antigen, which consists of a stretch of peptides from different parts of the JEV antigens.
The JE IgG ELISA consists of one enzymatically amplified “two-step” sandwich-type immunoassay.
In this assay, JE IgG Weak Positive Control (Represents reactive or equivocally reactive serum), JE Negative Control (Represents non-reactive serum), and unknown serum samples are incubated in microtitration wells. The serum samples may be diluted with Sample Dilution Buffer for IgG. After incubation and washing, the wells are treated with an antibody specific for human IgG and labeled with the enzyme horseradish peroxidase (HRP). After a second incubation and washing step, the wells are incubated with the tetramethylbenzidine (TMB) substrate.
Note: Depending on the strength of antibody response, sera can be diluted in the “Sample Dilution Buffer for JE IgG” provided in the kit.
An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. Above a certain threshold, the ratio of the absorbencies of the JERA and the control wells accurately determines whether antibodies to JEV are present.
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