Islet Cell Autoantibodies (ICA) ELISA
DRG´s Islet Cell Autoantibodies (ICA) ELISA is a qualitative ELISA test for measurement of circulating IgG antibodies against pancreatic islet cell antigens.
A purified mixture of pancreatic antigens is immobilized onto microwells. During an incubation period, antibodies in the serum sample are allowed to react at room temperature with antigen molecules on the microwells. After washing off excess/unbound serum materials, an enzyme (alkaline phosphatase) labeled goat antibody, specific to human IgG, is added to the antigen-antibody complex. After another thorough washing, a substrate (PNPP) is added and the color generated is measured spectrophotometrically. The intensity of the color is directly proportional to the concentration of ICA in the sample. An ICA-high-analyte control serves as an internal quality control and ensures valid results.
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