Irisin ELISA
Irisin is a recently described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin activates beige fat cells (beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone Irisin). Irisin is cleaved from the type I membrane protein FNDC5 and improves systemic metabolism by increasing energy expenditure. Circulating irisin levels were shown to be upregulated after acute exercise. Increase of irisin under conditions of obesity may indicate a physiological function to improve glucose tolerance.
Research topic
Energy metabolism and body weight regulation
Type
Competitive ELISA, Immobilized antigen
Applications
Serum, Plasma, Cell culture supernatant
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.001–5 μg/ml
Limit of Detection
1 ng/ml
– Belviranli M, Okudan N, Kabak B, Erdogan M, Karanfilci M. The relationship between brain-derived neurotrophic factor, irisin and cognitive skills of endurance athletes. Phys Sportsmed. 2016 Jun 2;
– Engin-Ustun Y, Caglayan EK, Gocmen AY, Polat MF. Postmenopausal Osteoporosis Is Associated with Serum Chemerin and Irisin but Not with Apolipoprotein M Levels. J Menopausal Med. 2016 Aug;22 (2):76-9
– Polyzos SA, Kountouras J, Anastasilakis AD, Geladari EV, Mantzoros CS. Irisin in patients with nonalcoholic fatty liver disease. Metabolism. 2014 Feb;63 (2):207-17