Interleukin-10 Rat ELISA
Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes.
The murine IL-10 exhibits strong DNA and aminoacid sequence homology to the human IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine’s biological activities and may therefore play a role in the host-virus interaction.
Research topic
Animal studies, Cytokines and chemokines and related molecules
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Cell culture supernatant
Sample Requirements
50 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
15.6–1000 pg/ml
Limit of Detection
1.5 pg/ml
Intra-assay (Within-Run)
CV = 5.0%
Inter-assay (Run-to-Run)
CV = 0,1%
Dilutation Linearity
112,00%
– Mahmoud MF, Gama Sl, Shaheen MA, El-Fayoumi HM. The effects of tramadol on hepatic ischemia/reperfusion injury in rats. Indian Journal of Pharmacology. 23-May-2016;48 (3):275-280