IgG anti U1-snRNP 68
Enzyme ImmunoAssay (ELISA) for the quantitative determination of IgG autoantibodies to RNP-68KDa in human plasma and sera.
Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease.
Rheumatoid autoimmune diseases are often associated with autoantibodies to Anti Nuclear Antibodies (ANA), associate with SLE (Systemic Lupus Erythematosus), RA (Reumatoid Arthritis), Scleroderma, MCDT (Mixed Connective Tissue Disease) and Sjogren’s Syndrome; and Extractable Nuclear Antibodies (ENA), associate with Polymyositis, SLE, MCDT and Sjogren’s Syndrome.
Up to 100% MCTD patients manifest high titers of Anti-RNP- 68. Often SLE typical autoantibodies (Anti-dsDNA, Anti-Sm) come along in the sera together with Anti-RNP-68. In these cases classical MCTD will proceed to SLE.
In ELISA Dia.Pro test the well are exclusively coated with Rec 68KDa protein of the U1-snRNP complex and so this assay provides the highly specific detection of mainly MCTD associated to Anti-RNP-68 autoantibodies.
Microplates are coated with a preparation of recombinant U1- snRNP 68 antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti U1-snRNP 68 IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti U1-snRNP 68 are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti U1-snRNP 68 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.
- LA Rokeach, JA Haselby, JF Meilof, RJ Smeenk, TR Unnasch, BM Greene and SO Hoch Characterization of the autoantigen calreticulin.The Journal of Immunology, Vol 147, Issue 9 3031-3039, Copyright © 1991 by American Association of Immunologists.
- Michael Mahler, Marvin J. Fritzler, Martin Bluthner. Arthritis res Ther 2005.Identification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti Sm-antibodies.