IgG anti SSB
The device SSB.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against SSB autoantigens in human plasma and sera.
Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease.
Rheumatoid autoimmune diseases are often associated with the occurrence of autoantibodies against several nuclear or cytoplasmatic antigens. We can to distinguish between Anti Nuclear Antibodies (ANA), associate with autoimmune systemic diseases as SLE (Systemic Lupus Erythematosus), RA (Reumatoid Arthritis), Scleroderma, MCDT (Mixed Connective Tissue Disease) and Sjögren’s Syndrome; and Extractable anti Nuclear Antibodies (ENA), associate with Polymyositis, SLE, MCDT and Sjogren’s Syndrome.
Ro/SSA and La/SSB are cytoplasmatic antigens that occur in combination in patient with Sjögren-Syndrome.
Autoantibodies against SSB ribonucleoprotein are present in approximately 60% of such patients.
Anti La/SSB are also associated with Systemic Lupus Erythematosus and they are present in most cases in the serum of mother’s children with neonatal SLE.
Microplates are coated with a preparation of recombinant SSB antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-SSB IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti-SSB are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-SSB IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.
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