IgG anti SSA 60 KD
The device SSA60.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against SSA-60KD autoantigens in human plasma and sera.
Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease.
Rheumatoid autoimmune diseases are often associated with the occurrence of autoantibodies against several nuclear or cytoplasmatic antigens. We can to distinguish between Anti Nuclear Antibodies (ANA), associate with autoimmune systemic diseases as SLE (Systemic Lupus Erythematosus), RA (Reumatoid Arthritis), Scleroderma, MCDT (Mixed Connective Tissue Disease) and Sjogren’s Syndrome; and Extractable anti Nuclear Antibodies (ENA), associate with Polymyositis, SLE, MCDT and Sjogren’s Syndrome.
Ro/SSA and La/SSB are cytoplasmatic antigens that occur in combination in patients with Sjögren-Syndrome. Autoantibodies against SSA-60 KDa ribonucleoprotein are clinically important since they are associated with subacute cutaneous lupus erythematosus (SCLE) and neonatal lupus erythematosus (NLE).
Anti-Ro/SSA are present in approximately 70% of such patients.
Microplates are coated with a preparation of recombinant SSA 60KDa antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-SSA60 IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti-SSA60 are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-SSA60 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.
- Characterization of murine monoclonal antibodies against 60-kD Ro/SS-A and La/SS-B autoantigens.C H Veldhoven, G J Pruijn, J F Meilof, J P Thijssen, A W Van der Kemp, W J Van Venrooij, and R J Smeenk. Clin Exp Immunol. 1995 July; 101(1): 45–54.
- Autoantibody Repertoire to Ro/SSA and La/SSB Antigens in Patients with Primary and Secondary Sjögren’s syndrome . Marie Wahren, Ludmila Solomin, Ingvar Pettersson and David Isenberg. Journal of Autoimmunity Volume 9, Issue 4, August 1996, Pages 537-544
- Specificity of autoantibodies for recombinant 60-kd and 52-kd ro autoantigens. E. ST. WilliamsClair, MD *, James A. Burch Jr., BS, Michael Saitta, MD. Arthritis and Rheumatism, Volume 37, Issue 9, Pages 1373- 1379.