IgG anti Jo-1
The device JO1.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Jo-1 autoantigens in human plasma and sera.
Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease.
Rheumatoid autoimmune diseases are often associated with autoantibodies to Nuclear Antigens. We can to distinguish between Anti Nuclear Antibodies (ANA) and Extractable anti Nuclear Antibodies (ENA) as Jo-1.
Anti-Jo-1 antibodies is directed at the cellular enzyme histidyl-t-RNA synthetase and is present in 35% of patients with Polymyositis and less commonly in dermatomyositis. Anti-Jo-1 autoantibodies are rare in other rheumatic diseases.
Microplates are coated with a preparation of recombinant Jo- 1 antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti Jo-1 IgG are captured, if present, by the solid phase.
After washing out all the other components of the sample, in the 2nd incubation bound anti Jo-1 IgG are detected by the addition of anti hIgG antibody, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti Jo-1 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.
- Arthritis&Rheumatism, volume 56 Issue 9, Pages 3125-3131. Anti- Jo-1 antibody levels correlate with disease activity in idiopathic inflammatory myopathy. KerryStone, Chester V. Oddis, Noreen Fertig, Yasuhiro Katsumata, Mary Lucas,Molly Vogt, Robyn Domsic, Dana P. Ascherman.
- Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells. O M Zack Howard, Hui Fang Dong, De Yang, Nina Raben, Kanneboyina Nagaraju, Antony Rosen, Livia Casciola-Rosen, Michael Härtlein, Michael Kron, David Yang, Kwabena Yiadom, Sunita Dwivedi, Paul H Plotz, Joost J Oppenheim. J. Exp. Med Sep 2002 (Vol. 196, Issue 6, Pages 781- 91).
- Anti-Jo-1 autoantibodies and the immunopathogenesis of autoimmune myositis. F W Tsui, K A Siminovitch. Int Rev Immunol 1991 (Vol. 7, Issue 3, Pages 225-35).
- Improved detection of anti-Jo-1 antibody, a marker for myositis, using purified histidyl-tRNA synthetase. E J Walker, K E Tymms, J Webb, P D Jeffrey. J Immunol Methods Feb 1987 (Vol. 96, Issue 2, Pages 149-56).